FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1793-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1793-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26622433
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11802234.433189859093645Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG867630.3259018437571051No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1312700.043.5612545
GGTCGCC1359550.041.997444
GTGGTCG1388950.041.02096642
TGGTCGC1389650.041.00030543
CTCGGTG1409000.040.5566238
TCTCGGT1418250.040.35148237
GGTGGTC1419900.040.12319641
ATCTCGG1431350.040.0372736
CGGTGGT1426450.039.9815440
TCGGTGG1433350.039.89888439
GATCTCG1482500.038.6167135
GCACACG145050.038.60834518
AGATCTC1546100.037.2813934
TAGATCT1612950.035.7502533
CACACGT109150.035.53775819
AGTGTAG1635650.034.79492629
GAGTGTA1649400.034.63003528
AGCACAC184100.034.21967317
GTGTAGA1665600.034.213930
GTAGATC1670950.034.11108832
Produced by FastQC (version 0.11.5)