FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1793-00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1793-00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19752440
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6881423.48383288343111Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG883470.4472713244540928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG780850.043.3915445
GGTCGCC808550.041.80481744
GTGGTCG826700.040.7672542
CTCGGTG833450.040.6417338
TGGTCGC832200.040.5059343
TCTCGGT842000.040.29770337
ATCTCGG847550.040.10549536
TCGGTGG855950.039.65994639
GGTGGTC851100.039.62169341
CGGTGGT855600.039.4343440
GATCTCG874900.038.75958335
GCACACG84200.038.2386918
AGATCTC915800.037.2324834
TAGATCT948700.035.97686833
AGTGTAG966250.034.96052629
GAGTGTA972700.034.9086828
CACACGT69400.034.62523319
GTAGATC981600.034.49633832
GTGTAGA988500.034.24188630
TGTAGAT999350.033.90839831
Produced by FastQC (version 0.11.5)