FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1792-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1792-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23227459
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG6490402.7942789609487635Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG683070.2940786592282867No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG730650.043.11766445
GGTCGCC753150.041.71611844
GTGGTCG774650.040.543742
CTCGGTG779850.040.34802238
TGGTCGC781050.040.18276243
TCTCGGT788700.039.98596237
TCGGTGG800800.039.3795639
GGTGGTC797650.039.37147541
ATCTCGG803850.039.27450636
CGGTGGT812000.038.7643540
GCACACG146400.037.99094418
GATCTCG855300.036.87801435
GAGCACA240650.035.03237516
AGCACAC185100.035.0072417
AGAGCAC244000.034.90180215
CACACGT108050.034.54602419
AGATCTC928850.034.1468134
TAGATCT989150.032.08798233
ACACGTC87500.032.0138420
AGTGTAG1037100.030.2962829
Produced by FastQC (version 0.11.5)