FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1792-05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1792-05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18398365
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10904315.926782080907733Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1355870.736951354101302No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG195580.106302924200058Illumina Single End PCR Primer 1 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1237000.043.8665845
GGTCGCC1284850.042.23466544
GTGGTCG1301150.041.46694642
TGGTCGC1305850.041.317743
GGTGGTC1318750.040.90603641
CTCGGTG1324550.040.6674638
CGGTGGT1327450.040.65997740
TCTCGGT1333450.040.44386337
TCGGTGG1340750.040.25652739
ATCTCGG1354600.039.8956636
GCACACG138250.039.6447218
GATCTCG1424500.037.90028435
CACACGT95550.036.19260819
AGATCTC1507300.036.10342434
GAGCACA234250.035.9895216
AGCACAC176950.035.97115317
AGAGCAC241200.035.20446415
ACACGTC73400.034.4545820
TAGATCT1585350.034.3032733
AGTGTAG1641150.032.64632429