Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n02_ds1792-05.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 18398365 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1090431 | 5.926782080907733 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 135587 | 0.736951354101302 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 19558 | 0.106302924200058 | Illumina Single End PCR Primer 1 (98% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 123700 | 0.0 | 43.86658 | 45 |
GGTCGCC | 128485 | 0.0 | 42.234665 | 44 |
GTGGTCG | 130115 | 0.0 | 41.466946 | 42 |
TGGTCGC | 130585 | 0.0 | 41.3177 | 43 |
GGTGGTC | 131875 | 0.0 | 40.906036 | 41 |
CTCGGTG | 132455 | 0.0 | 40.66746 | 38 |
CGGTGGT | 132745 | 0.0 | 40.659977 | 40 |
TCTCGGT | 133345 | 0.0 | 40.443863 | 37 |
TCGGTGG | 134075 | 0.0 | 40.256527 | 39 |
ATCTCGG | 135460 | 0.0 | 39.89566 | 36 |
GCACACG | 13825 | 0.0 | 39.64472 | 18 |
GATCTCG | 142450 | 0.0 | 37.900284 | 35 |
CACACGT | 9555 | 0.0 | 36.192608 | 19 |
AGATCTC | 150730 | 0.0 | 36.103424 | 34 |
GAGCACA | 23425 | 0.0 | 35.98952 | 16 |
AGCACAC | 17695 | 0.0 | 35.971153 | 17 |
AGAGCAC | 24120 | 0.0 | 35.204464 | 15 |
ACACGTC | 7340 | 0.0 | 34.45458 | 20 |
TAGATCT | 158535 | 0.0 | 34.30327 | 33 |
AGTGTAG | 164115 | 0.0 | 32.646324 | 29 |