Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n02_ds1792-04.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23429744 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1683091 | 7.183565471308606 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 122216 | 0.5216275517137533 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 30354 | 0.12955327211428344 | Illumina Single End PCR Primer 1 (98% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 186840 | 0.0 | 43.731453 | 45 |
GGTCGCC | 193190 | 0.0 | 42.25569 | 44 |
GTGGTCG | 196070 | 0.0 | 41.500664 | 42 |
TGGTCGC | 196315 | 0.0 | 41.4524 | 43 |
GGTGGTC | 198320 | 0.0 | 41.016735 | 41 |
CTCGGTG | 199335 | 0.0 | 40.900608 | 38 |
CGGTGGT | 199430 | 0.0 | 40.786182 | 40 |
TCTCGGT | 200425 | 0.0 | 40.731186 | 37 |
TCGGTGG | 201010 | 0.0 | 40.604385 | 39 |
GCACACG | 25940 | 0.0 | 40.228676 | 18 |
ATCTCGG | 203565 | 0.0 | 40.206886 | 36 |
GATCTCG | 212935 | 0.0 | 38.409176 | 35 |
GAGCACA | 42405 | 0.0 | 38.27127 | 16 |
AGCACAC | 31675 | 0.0 | 38.25813 | 17 |
AGAGCAC | 42955 | 0.0 | 37.927982 | 15 |
CACACGT | 15695 | 0.0 | 37.33011 | 19 |
AGATCTC | 224215 | 0.0 | 36.714672 | 34 |
CACACGA | 12185 | 0.0 | 36.59803 | 19 |
ACACGAG | 12270 | 0.0 | 35.409294 | 20 |
ACACGTC | 11405 | 0.0 | 35.39213 | 20 |