FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1792-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1792-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23429744
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG16830917.183565471308606Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1222160.5216275517137533No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG303540.12955327211428344Illumina Single End PCR Primer 1 (98% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1868400.043.73145345
GGTCGCC1931900.042.2556944
GTGGTCG1960700.041.50066442
TGGTCGC1963150.041.452443
GGTGGTC1983200.041.01673541
CTCGGTG1993350.040.90060838
CGGTGGT1994300.040.78618240
TCTCGGT2004250.040.73118637
TCGGTGG2010100.040.60438539
GCACACG259400.040.22867618
ATCTCGG2035650.040.20688636
GATCTCG2129350.038.40917635
GAGCACA424050.038.2712716
AGCACAC316750.038.2581317
AGAGCAC429550.037.92798215
CACACGT156950.037.3301119
AGATCTC2242150.036.71467234
CACACGA121850.036.5980319
ACACGAG122700.035.40929420
ACACGTC114050.035.3921320