FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1792-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1792-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25694918
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG313609612.205121650903887Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2084910.8114094779364542No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG585250.22776877513288815Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT378820.14742993147516562Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG362040.1408994572389762Illumina Single End PCR Primer 1 (100% over 41bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC331830.1291422685217365Illumina Single End PCR Primer 1 (100% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC303230.11801166285099644Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG3461100.043.96000745
GGTCGCC3573550.042.53766644
TGGTCGC3617600.041.93760743
GTGGTCG3619000.041.93054642
GGTGGTC3641700.041.6545641
CTCGGTG3662150.041.52439538
CGGTGGT3656450.041.49514440
TCGGTGG3680050.041.36460539
TCTCGGT3680150.041.35680437
ATCTCGG3745400.040.6927836
GCACACG202850.038.97616618
GATCTCG3926200.038.7279335
GAGCACA382950.037.71357316
AGAGCAC392000.037.14135715
AGATCTC4141550.036.90594534
AGCACAC267500.036.79820617
CACACGA105850.035.4766119
TAGATCT4330650.035.30547333
GTGACTG166150.034.598261
TGACTGG167050.034.4661672