Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n02_ds1792-03.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25694918 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 3136096 | 12.205121650903887 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 208491 | 0.8114094779364542 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 58525 | 0.22776877513288815 | Illumina Single End PCR Primer 1 (98% over 51bp) |
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 37882 | 0.14742993147516562 | Illumina Single End PCR Primer 1 (100% over 39bp) |
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG | 36204 | 0.1408994572389762 | Illumina Single End PCR Primer 1 (100% over 41bp) |
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 33183 | 0.1291422685217365 | Illumina Single End PCR Primer 1 (100% over 50bp) |
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 30323 | 0.11801166285099644 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 346110 | 0.0 | 43.960007 | 45 |
GGTCGCC | 357355 | 0.0 | 42.537666 | 44 |
TGGTCGC | 361760 | 0.0 | 41.937607 | 43 |
GTGGTCG | 361900 | 0.0 | 41.930546 | 42 |
GGTGGTC | 364170 | 0.0 | 41.65456 | 41 |
CTCGGTG | 366215 | 0.0 | 41.524395 | 38 |
CGGTGGT | 365645 | 0.0 | 41.495144 | 40 |
TCGGTGG | 368005 | 0.0 | 41.364605 | 39 |
TCTCGGT | 368015 | 0.0 | 41.356804 | 37 |
ATCTCGG | 374540 | 0.0 | 40.69278 | 36 |
GCACACG | 20285 | 0.0 | 38.976166 | 18 |
GATCTCG | 392620 | 0.0 | 38.72793 | 35 |
GAGCACA | 38295 | 0.0 | 37.713573 | 16 |
AGAGCAC | 39200 | 0.0 | 37.141357 | 15 |
AGATCTC | 414155 | 0.0 | 36.905945 | 34 |
AGCACAC | 26750 | 0.0 | 36.798206 | 17 |
CACACGA | 10585 | 0.0 | 35.47661 | 19 |
TAGATCT | 433065 | 0.0 | 35.305473 | 33 |
GTGACTG | 16615 | 0.0 | 34.59826 | 1 |
TGACTGG | 16705 | 0.0 | 34.466167 | 2 |