FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1792-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1792-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23026489
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG237805510.327475456636051Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1798730.7811568667720034No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG474140.2059106796524646Illumina Single End PCR Primer 1 (98% over 51bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT277260.12040915139081777Illumina Single End PCR Primer 1 (100% over 39bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG269500.11703911959830263Illumina Single End PCR Primer 1 (100% over 41bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC258200.11213172794167622Illumina Single End PCR Primer 1 (100% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC235260.10216928859627711Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG2627000.044.00612345
GGTCGCC2718850.042.505544
GTGGTCG2757100.041.86113442
TGGTCGC2758850.041.81908843
GGTGGTC2776300.041.55074341
CTCGGTG2786950.041.4861538
CGGTGGT2787400.041.3933540
TCGGTGG2804050.041.29413639
TCTCGGT2803200.041.2905137
GCACACG284700.040.96050318
ATCTCGG2846050.040.72514336
GAGCACA461000.039.3079416
AGCACAC344600.039.03758217
GATCTCG2969800.038.97385835
AGAGCAC467500.038.89144515
CACACGT172550.038.3231219
CACACGA130700.037.7519419
AGATCTC3106550.037.47405634
ACACGTC121000.036.4829420
TAGATCT3230700.036.0501733