Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n02_ds1792-02.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23026489 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 2378055 | 10.327475456636051 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 179873 | 0.7811568667720034 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 47414 | 0.2059106796524646 | Illumina Single End PCR Primer 1 (98% over 51bp) |
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 27726 | 0.12040915139081777 | Illumina Single End PCR Primer 1 (100% over 39bp) |
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG | 26950 | 0.11703911959830263 | Illumina Single End PCR Primer 1 (100% over 41bp) |
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 25820 | 0.11213172794167622 | Illumina Single End PCR Primer 1 (100% over 50bp) |
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 23526 | 0.10216928859627711 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 262700 | 0.0 | 44.006123 | 45 |
GGTCGCC | 271885 | 0.0 | 42.5055 | 44 |
GTGGTCG | 275710 | 0.0 | 41.861134 | 42 |
TGGTCGC | 275885 | 0.0 | 41.819088 | 43 |
GGTGGTC | 277630 | 0.0 | 41.550743 | 41 |
CTCGGTG | 278695 | 0.0 | 41.48615 | 38 |
CGGTGGT | 278740 | 0.0 | 41.39335 | 40 |
TCGGTGG | 280405 | 0.0 | 41.294136 | 39 |
TCTCGGT | 280320 | 0.0 | 41.29051 | 37 |
GCACACG | 28470 | 0.0 | 40.960503 | 18 |
ATCTCGG | 284605 | 0.0 | 40.725143 | 36 |
GAGCACA | 46100 | 0.0 | 39.30794 | 16 |
AGCACAC | 34460 | 0.0 | 39.037582 | 17 |
GATCTCG | 296980 | 0.0 | 38.973858 | 35 |
AGAGCAC | 46750 | 0.0 | 38.891445 | 15 |
CACACGT | 17255 | 0.0 | 38.32312 | 19 |
CACACGA | 13070 | 0.0 | 37.75194 | 19 |
AGATCTC | 310655 | 0.0 | 37.474056 | 34 |
ACACGTC | 12100 | 0.0 | 36.48294 | 20 |
TAGATCT | 323070 | 0.0 | 36.05017 | 33 |