FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1792-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1792-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18262683
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG16684279.135716805685124Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1835771.0052027952300326No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG280570.15363021961230997Illumina Single End PCR Primer 1 (98% over 51bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC189890.1039770552880976Illumina Single End PCR Primer 1 (100% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC184570.10106401124084562Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1843050.043.94959345
GGTCGCC1907500.042.4247744
GTGGTCG1936800.041.69920742
TGGTCGC1940450.041.6162543
GGTGGTC1953300.041.348741
CTCGGTG1961550.041.306738
CGGTGGT1960450.041.2151140
TCTCGGT1972050.041.1511337
GCACACG256900.041.0670618
TCGGTGG1975250.041.0609939
ATCTCGG1998300.040.6793536
GAGCACA429750.039.7530316
AGCACAC310350.039.45295317
AGAGCAC437750.039.16016415
GATCTCG2072800.039.1193635
CACACGA129950.038.6800219
CACACGT142500.038.14713319
AGATCTC2162150.037.7015234
ACACGAG133450.037.21033520
ACACGTC98600.036.94449620