Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n02_ds1792-01.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 18262683 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1668427 | 9.135716805685124 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 183577 | 1.0052027952300326 | No Hit |
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 28057 | 0.15363021961230997 | Illumina Single End PCR Primer 1 (98% over 51bp) |
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 18989 | 0.1039770552880976 | Illumina Single End PCR Primer 1 (100% over 50bp) |
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 18457 | 0.10106401124084562 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 184305 | 0.0 | 43.949593 | 45 |
GGTCGCC | 190750 | 0.0 | 42.42477 | 44 |
GTGGTCG | 193680 | 0.0 | 41.699207 | 42 |
TGGTCGC | 194045 | 0.0 | 41.61625 | 43 |
GGTGGTC | 195330 | 0.0 | 41.3487 | 41 |
CTCGGTG | 196155 | 0.0 | 41.3067 | 38 |
CGGTGGT | 196045 | 0.0 | 41.21511 | 40 |
TCTCGGT | 197205 | 0.0 | 41.15113 | 37 |
GCACACG | 25690 | 0.0 | 41.06706 | 18 |
TCGGTGG | 197525 | 0.0 | 41.06099 | 39 |
ATCTCGG | 199830 | 0.0 | 40.67935 | 36 |
GAGCACA | 42975 | 0.0 | 39.75303 | 16 |
AGCACAC | 31035 | 0.0 | 39.452953 | 17 |
AGAGCAC | 43775 | 0.0 | 39.160164 | 15 |
GATCTCG | 207280 | 0.0 | 39.11936 | 35 |
CACACGA | 12995 | 0.0 | 38.68002 | 19 |
CACACGT | 14250 | 0.0 | 38.147133 | 19 |
AGATCTC | 216215 | 0.0 | 37.70152 | 34 |
ACACGAG | 13345 | 0.0 | 37.210335 | 20 |
ACACGTC | 9860 | 0.0 | 36.944496 | 20 |