Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n02_ds1792-00.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 19454992 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 1744222 | 8.965421317058366 | Illumina Single End PCR Primer 1 (100% over 51bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 162852 | 0.8370705061199716 | No Hit |
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 21185 | 0.1088923603772235 | Illumina Single End PCR Primer 1 (100% over 39bp) |
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 20999 | 0.1079363075554079 | Illumina Single End PCR Primer 1 (100% over 50bp) |
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 20712 | 0.10646110777120855 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG | 19654 | 0.10102291483851547 | Illumina Single End PCR Primer 1 (100% over 41bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTCGCCG | 189810 | 0.0 | 43.852097 | 45 |
GCACACG | 44110 | 0.0 | 42.325947 | 18 |
GGTCGCC | 197055 | 0.0 | 42.14493 | 44 |
GAGCACA | 76125 | 0.0 | 41.422485 | 16 |
GTGGTCG | 200480 | 0.0 | 41.3732 | 42 |
TGGTCGC | 201065 | 0.0 | 41.26524 | 43 |
CACACGA | 22155 | 0.0 | 41.130047 | 19 |
AGCACAC | 52735 | 0.0 | 41.06076 | 17 |
GGTGGTC | 202220 | 0.0 | 41.006767 | 41 |
CGGTGGT | 203510 | 0.0 | 40.74794 | 40 |
AGAGCAC | 77815 | 0.0 | 40.64141 | 15 |
CTCGGTG | 205845 | 0.0 | 40.466953 | 38 |
ACACGAG | 22290 | 0.0 | 40.396423 | 20 |
TCTCGGT | 207135 | 0.0 | 40.290344 | 37 |
TCGGTGG | 207080 | 0.0 | 40.279938 | 39 |
ATCTCGG | 209685 | 0.0 | 39.834705 | 36 |
CACACGT | 23745 | 0.0 | 39.787773 | 19 |
CACGAGA | 22975 | 0.0 | 39.211086 | 21 |
AAGAGCA | 113580 | 0.0 | 39.083687 | 14 |
ACACGTC | 16125 | 0.0 | 38.469185 | 20 |