FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n02_ds1792-00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n02_ds1792-00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19454992
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG17442228.965421317058366Illumina Single End PCR Primer 1 (100% over 51bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1628520.8370705061199716No Hit
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT211850.1088923603772235Illumina Single End PCR Primer 1 (100% over 39bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC209990.1079363075554079Illumina Single End PCR Primer 1 (100% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC207120.10646110777120855Illumina Single End PCR Primer 1 (100% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG196540.10102291483851547Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG1898100.043.85209745
GCACACG441100.042.32594718
GGTCGCC1970550.042.1449344
GAGCACA761250.041.42248516
GTGGTCG2004800.041.373242
TGGTCGC2010650.041.2652443
CACACGA221550.041.13004719
AGCACAC527350.041.0607617
GGTGGTC2022200.041.00676741
CGGTGGT2035100.040.7479440
AGAGCAC778150.040.6414115
CTCGGTG2058450.040.46695338
ACACGAG222900.040.39642320
TCTCGGT2071350.040.29034437
TCGGTGG2070800.040.27993839
ATCTCGG2096850.039.83470536
CACACGT237450.039.78777319
CACGAGA229750.039.21108621
AAGAGCA1135800.039.08368714
ACACGTC161250.038.46918520