FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25403130
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC19311697.602090765980413TruSeq Adapter, Index 14 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT753590.2966524203907156TruSeq Adapter, Index 14 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTG739870.2912515111326832TruSeq Adapter, Index 14 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACAC604630.2380139770177927TruSeq Adapter, Index 13 (97% over 35bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT529320.20836802394035694TruSeq Adapter, Index 14 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACACG460290.18119420717053372Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC429650.16913270136396577TruSeq Adapter, Index 14 (97% over 36bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACG402490.15844110548582008TruSeq Adapter, Index 14 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCCACAGATCGGAAGAGCACAC398070.15670116241581256TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCACCCACAGATCGGAAGAGCACAC368520.14506873759257227Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC358590.14115977046922956TruSeq Adapter, Index 14 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACAGATCGGAAGAGCACAC301260.11859168535530858TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCATTCACAGATCGGAAGAGCACAC292840.1152771331721721TruSeq Adapter, Index 13 (97% over 35bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC284230.11188778705616198TruSeq Adapter, Index 14 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG152150.035.824631
TGACTGG152300.034.4151082
ACTGGAG152950.032.8622934
GTCGGAG38750.032.116852
AGTCGGA204450.031.1514931
CCAGTCA3588650.030.35770427
TCCAGTC3658500.029.97243526
CAGTCAC3599150.029.95963928
CTCCAGT3724100.029.7917325
GGAGTTC169800.029.4819667
TCACAGA3380700.029.41067731
ACTCCAG3912450.029.39037924
GTCACAG3201100.029.31432230
CACAGAT3575350.029.2568932
AACTCCA4282150.029.03094723
ACTCAGT77600.028.99481824
GAACTCC4404000.028.9097722
ACAGATC3633300.028.87385233
AGTCACA3337450.028.7830429
AGTCACT111700.028.30072429
Produced by FastQC (version 0.11.5)