FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds2124-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds2124-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18813450
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC5163892.7447863097943226TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATGC412010.2189975788598051TruSeq Adapter, Index 22 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC667700.038.20604345
CATCTCG647550.037.8420740
CTCGTAT646400.037.30711743
ATCTCGT659800.036.86326641
TCGTATG644200.036.83349244
GCATCTC677650.036.14127339
TCTCGTA674050.035.94699542
AGCATCT709650.034.61936638
TAGCATC798450.033.2376837
GTAGCAT798050.033.22887836
GGTAGCA822650.032.2242835
CGGTAGC834700.031.74273734
AGTCGGA48100.031.5825861
CACGGTA875150.030.3912632
GTCACGG876650.030.33925830
AGTCACG886400.030.0843929
TCACGGT884200.030.07510831
ACGGTAG894350.029.69353533
CAGTCAC946350.028.24039828
CCAGTCA959400.027.95476527
Produced by FastQC (version 0.11.5)