FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds2123-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds2123-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17590204
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC6900213.9227572346517414TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATGC575050.3269149124137503TruSeq Adapter, Index 7 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC869250.039.07467345
GATCTCG824500.038.27837840
CTCGTAT827750.037.99761243
TCGTATG829250.037.7333144
ATCTCGT836000.037.7114541
GGATCTC839650.037.55019439
TCTCGTA850250.037.0942
TGGATCT863350.036.58194738
GTGGATC957000.035.85867737
AGTGGAT1000750.034.3247636
GAGTGGA1009200.034.02844235
CGAGTGG1025850.033.46937634
ACGAGTG1051300.032.76196333
CACGAGT1060850.032.51369532
TCACGAG1073000.032.166531
GTCACGA1080050.032.00444830
AGTCACG1085000.031.88124829
AGTCGGA42550.030.6234041
CAGTCAC1139100.030.43227228
CCAGTCA1151650.030.14166827
Produced by FastQC (version 0.11.5)