FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds2113-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds2113-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12884274
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC1957011.5189136772471619TruSeq Adapter, Index 22 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATGC141710.10998679475459773TruSeq Adapter, Index 22 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG251050.036.6199240
CGTATGC266500.036.10090345
CTCGTAT253300.035.73502743
CGATCTC258400.035.58739
ATCTCGT259250.035.23599641
ACGATCT267200.034.5074738
TCTCGTA264500.034.4175142
TACGATC293050.033.98954437
TCGTATG264050.033.8625244
CGTACGA293950.033.82411235
CCGTACG297400.033.4241734
GTACGAT299900.033.19042636
ACCGTAC319900.031.19298633
CACCGTA326750.030.59414332
GTCACCG327350.030.55868530
AGTCACC329850.030.38188429
TCACCGT339700.029.49407231
CAGTCAC343850.029.30834228
CCAGTCA345800.029.12355427
TCCAGTC352200.028.67099626
Produced by FastQC (version 0.11.5)