FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds2102-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds2102-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18307636
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1938921.059077206909729TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGGATGC197330.10778562562637799TruSeq Adapter, Index 4 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC193060.10545326551172418TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT279450.035.8694543
AATCTCG348000.034.4029140
ATCTCGT320000.034.05223541
CGTATGC288150.033.70145
TCTCGTA303900.033.31677642
TCGTATG287800.031.95941744
CAATCTC377950.031.73028639
CCAATCT385100.031.15276338
GACCAAT420150.030.82435836
ACCAATC420900.030.78563537
AGTCACT447500.029.27229329
ACTGACC448750.029.01025633
CAGTCAC456350.028.84266728
CTGACCA454950.028.55061734
CCAGTCA465850.028.27380427
TCCAGTC474400.027.90651126
CACTGAC469800.027.8349332
TGACCAA473350.027.45981435
GTCACTG485900.026.93579330
TCACTGA492800.026.57690831
Produced by FastQC (version 0.11.5)