FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds2098-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds2098-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19421180
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC937730.4828388388347155TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG141800.032.22664640
CGTATGC166300.029.87325745
GCATCTC154300.029.52843939
CTTAGGC167900.029.07935734
CTCGTAT159900.028.6910743
AGGCATC172050.028.62677837
ACTTAGG171650.028.6013633
TAGGCAT172650.028.39682436
ATCTCGT163000.028.14563241
TTAGGCA173800.028.14420135
GGCATCT163500.027.81185538
TCGTATG169850.027.06316644
TCTCGTA171700.027.02068942
CACTTAG194550.025.40824132
CAGTCAC199700.025.16993128
AGTCACT200500.024.92361629
CCAGTCA208200.024.2720227
TCCAGTC211350.024.00607726
TCACTTA210000.023.83891731
GAACTCC229100.023.24627922
Produced by FastQC (version 0.11.5)