FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds2092-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds2092-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14609290
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC5220633.5735001495623675TruSeq Adapter, Index 21 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCGCGTATGC415560.28444914160783996TruSeq Adapter, Index 21 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC667750.039.50372345
GATCTCG630400.038.76449640
CTCGTAT635500.038.43924743
ATCTCGT642950.037.9903441
TCGTATG642500.037.92551444
TCGATCT649900.037.59792338
CGATCTC649800.037.57254839
TCTCGTA653950.037.42356542
TTCGATC742250.035.6998637
CGTTTCG766800.034.5682734
GTTTCGA778450.034.091435
GTCACGT803750.033.1385530
AGTCACG806250.033.12788429
ACGTTTC802900.033.11500533
TTTCGAT803550.033.05450436
CACGTTT812250.032.7670532
TCACGTT826100.032.2340431
CAGTCAC846400.031.61224428
CCAGTCA854100.031.3878427
TCCAGTC866950.031.01603726
Produced by FastQC (version 0.11.5)