FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds2089-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds2089-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17722726
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2529951.4275174146460312TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATGC198760.11214979004922832TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG335650.035.8633240
CGTATGC367350.035.52437245
CTCGTAT346700.034.74624643
ATCTCGT355000.033.97189341
TCGTATG359700.033.6088544
TCTCGTA364350.033.19891442
GTATCTC387200.031.15256539
CGATGTA428300.030.2484635
ATGTATC431450.030.1217437
CCGATGT431100.030.0413934
TGTATCT408450.029.55936838
ACCGATG442050.029.4244833
GATGTAT443700.029.2290236
CACCGAT447000.029.12884332
GTCACCG448400.029.0879930
AGTCACC453050.028.89869729
TCACCGA453300.028.83320431
CAGTCAC467300.028.1281928
CCAGTCA479750.027.50156627
TCCAGTC491100.027.0858826
Produced by FastQC (version 0.11.5)