FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds2086-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds2086-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22540348
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC535040.2373698933130935TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG100950.027.5258140
CACGATC115950.026.0994937
CGATCTC109600.025.29179439
CATCACG126000.024.01758234
TCACGAT126800.023.93713836
CTCGTAT111150.023.92690543
AGTCACA157300.023.8871529
CAGTCAC158300.023.82153328
ACGATCT117750.023.80875238
ATCACGA129600.023.4894235
CCAGTCA163150.023.26508327
GAACTCC176800.022.98343822
TCCAGTC166450.022.9795626
GTCACAT134350.022.84295730
ATCTCGT119600.022.70678141
CGTATGC123900.022.40889545
ACTCCAG178100.021.81764824
ACACGTC194750.021.19992314
ACGTCTG196500.020.87371416
CACACGT199000.020.8036913
Produced by FastQC (version 0.11.5)