FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1793-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1793-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24017265
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC4175381.7384910396750004TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC294120.12246190396783316TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC525900.039.41620345
GATCTCG508100.038.2956740
TCGTATG512750.037.86437244
CTCGTAT518500.037.4796543
GTGATCT527100.036.8640338
ATCTCGT527950.036.84729441
AGTGATC574600.036.12737
TCTCGTA540150.036.1023842
TGATCTC548400.035.4732539
CAGTGAT610850.034.00491336
CACAGTG630600.032.96103734
ACAGTGA632700.032.866235
ACACAGT665950.031.25200533
CACACAG667450.031.23907532
GTCACAC688300.030.3057330
AGTCACA729150.028.70371429
TCACACA734400.028.56586331
CAGTCAC737650.028.39730528
CCAGTCA760250.027.56497227
TCCAGTC773250.027.19465826
Produced by FastQC (version 0.11.5)