FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1793-05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1793-05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24999949
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC8710963.4843911081578605TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC638880.2555525213271435TruSeq Adapter, Index 6 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1065900.040.26680845
TATCTCG1016950.039.32485640
TCGTATG1026150.039.06171444
CTCGTAT1033450.038.8188443
ATCTCGT1040650.038.52655441
TCTCGTA1051850.038.15903542
ATATCTC1093100.036.65941639
GCCAATA1234200.034.78716335
AATATCT1163950.034.559438
CAATATC1252350.034.3585337
CCAATAT1253050.034.33201236
CGCCAAT1257050.034.17595734
CACGCCA1298200.033.2417732
ACGCCAA1298900.033.24117733
TCACGCC1308750.032.95317531
GTCACGC1320550.032.67746430
AGTCACG1347300.032.09899529
CAGTCAC1414550.030.64288328
CCAGTCA1442950.030.06790227
TCCAGTC1467700.029.61298226
Produced by FastQC (version 0.11.5)