FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1793-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1793-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22817593
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC10680884.680984536800178TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC834460.365709038635232TruSeq Adapter, Index 7 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1310900.040.955745
CATCTCG1238150.040.24252740
TCGTATG1245850.039.96257844
CTCGTAT1252500.039.8207243
ATCTCGT1260300.039.56025341
TCTCGTA1268550.039.3399642
TCATCTC1318950.037.78405439
GATCATC1450450.037.12436337
AGATCAT1466200.036.72993536
CAGATCA1492800.036.08449635
ATCATCT1396600.035.8572138
CCAGATC1666700.032.59184634
ACCAGAT1680950.032.36775633
TCACCAG1691150.032.1858431
CACCAGA1699900.032.0585532
AGTCACC1710600.031.88322329
GTCACCA1722050.031.64873730
CAGTCAC1767550.031.01634428
CCAGTCA1803600.030.43132427
TCCAGTC1835150.029.97067626
Produced by FastQC (version 0.11.5)