FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1793-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1793-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24991169
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC15696706.280898664644299TruSeq Adapter, Index 8 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC2682851.0735192099257143TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC2669411.0681413102364279TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC2312890.925482917585808TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC1059830.4240818026559702TruSeq Adapter, Index 8 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTGC408500.1634577398120112TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTTC394570.15788377086321972TruSeq Adapter, Index 8 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATTC270900.10839829061217585TruSeq Adapter, Index 8 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTTTGC264220.1057253464213699TruSeq Adapter, Index 8 (97% over 46bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG255180.10210806865417141TruSeq Adapter, Index 8 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1820850.042.56386645
TCGTATG1759450.042.091444
CTCGTAT2077350.041.9022243
TCTCGTA2111350.041.57166342
ATCTCGT2482150.040.8664941
AATCTCG2930550.040.4302140
GAATCTC2984950.039.6594639
TGAATCT3017300.039.2923438
CGGATGC344400.039.1327345
CTCGGAT376400.038.496243
TCGGATG339100.038.39079744
TTGAATC3342900.038.067437
TCTCGGA388600.037.61765742
CTTGAAT3403400.037.3865836
ACTTGAA3506650.036.33389335
CGTATTC361850.036.27557845
CACTTGA3562050.035.80697334
ATCTCGG480000.035.69531641
AGTCGGA80200.035.161751
ACACTTG3690100.034.58272633
Produced by FastQC (version 0.11.5)