FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1793-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1793-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26871466
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10193013.7932467100976184TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC754700.28085553650106027TruSeq Adapter, Index 9 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1254100.040.30784645
GATCTCG1190600.039.56294340
TCGTATG1193900.039.2967344
CTCGTAT1196450.039.29608543
ATCTCGT1204250.039.0976941
TCTCGTA1217100.038.70516242
AGATCTC1219950.038.59267839
CAGATCT1250150.037.7107938
CGATCAG1397450.036.3807134
GATCAGA1403150.036.28443535
TCAGATC1404600.036.21360837
ATCAGAT1421200.035.84576436
CACGATC1464950.034.9118132
GTCACGA1476650.034.67931730
AGTCACG1486250.034.4750629
ACGATCA1480950.034.47081833
TCACGAT1486700.034.42376331
CAGTCAC1548450.033.16723628
CCAGTCA1574800.032.64233827
TCCAGTC1594050.032.3356626
Produced by FastQC (version 0.11.5)