FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1793-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1793-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26622433
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC11382024.275349289075119TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGC924460.34724850279461683TruSeq Adapter, Index 10 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1424250.040.14026345
TATCTCG1332350.039.6043540
TCGTATG1341850.039.36558544
CTCGTAT1347750.039.21510743
ATCTCGT1354900.039.00498641
TCTCGTA1365750.038.7246342
TTATCTC1409800.037.39509639
CTTATCT1411750.037.36568538
GCTTATC1529800.037.2517637
AGCTTAT1563600.036.4924136
TAGCTTA1583000.036.02670735
CTAGCTT1630700.034.9546834
ACTAGCT1666500.034.31461333
CACTAGC1675900.034.24163432
TCACTAG1701850.033.76181831
GTCACTA1726600.033.33650230
AGTCACT1726900.033.31383529
CAGTCAC1776350.032.47764228
CCAGTCA1805200.032.0022227
TCCAGTC1829650.031.67663626
Produced by FastQC (version 0.11.5)