FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1793-00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1793-00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19752440
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC6728463.4063943492550792TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATGC577080.29215631081527144TruSeq Adapter, Index 11 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC857650.039.61613545
CATCTCG801100.038.99508340
CTCGTAT815250.038.4450243
TCGTATG815550.038.4223144
ATCTCGT816700.038.31083741
TCTCGTA826650.037.91211742
ACATCTC835950.037.40978639
GCTACAT928750.036.5740236
GGCTACA930100.036.4822335
TACATCT869550.036.12467238
CGGCTAC946500.035.8472734
CTACATC949650.035.7881437
ACGGCTA970650.035.0249233
CACGGCT971250.035.01718532
TCACGGC980350.034.73804531
GTCACGG985950.034.609230
AGTCACG997500.034.3302629
CAGTCAC1042800.032.91660728
CCAGTCA1061750.032.36725627
TCCAGTC1074850.032.03557226
Produced by FastQC (version 0.11.5)