FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1792-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1792-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23227459
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6256582.693613623427341TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATGC436590.18796287618030022TruSeq Adapter, Index 12 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC782400.039.62178445
TCGTATG759550.038.23956344
CTCGTAT774750.037.78878443
AATCTCG786150.037.5098440
ATCTCGT783650.037.480241
TCTCGTA798900.036.7957342
TAATCTC811300.036.3498239
GTAATCT813350.036.29416738
TGTAATC881550.035.80891437
TTGTAAT968850.032.54956436
CCTTGTA994400.031.64981734
CTTGTAA996300.031.63476235
ACCTTGT1061100.029.6560933
CACCTTG1077250.029.30756832
GTCACCT1082300.029.20230
AGTCACC1090700.029.13387929
CAGTCAC1129100.028.18484128
TCACCTT1137150.027.7875231
CCAGTCA1158950.027.48032827
TCCAGTC1187000.026.90296726
Produced by FastQC (version 0.11.5)