FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1792-05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1792-05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18398365
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC3917252.1291294090534674TruSeq Adapter, Index 13 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATTC1442720.7841566356575705TruSeq Adapter, Index 13 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATGC1434910.7799116932401331TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTTTGC1324620.7199661491659721TruSeq Adapter, Index 13 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGTTGC498080.2707197079740509TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTTTTC463640.25200065331892263TruSeq Adapter, Index 13 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGTATGC315250.17134674738760752TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATTC300940.1635688823436213TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGG192350.1045473334179423TruSeq Adapter, Index 13 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC478950.040.94075845
CTCGTAT652200.040.38739443
TCGTATG479150.039.91407444
TCTCGTA676600.039.4463642
ATCTCGT927550.039.1125841
AATCTCG1261500.038.57715240
CGGATGC186300.038.41753445
CTCGGAT232250.038.04443
TCGGATG192500.037.53083444
TCTCGGA238950.037.4198342
AAATCTC1329350.036.6301839
CAAATCT1336550.036.49167338
GTCAAAT1506500.035.351436
ATCTCGG352850.035.23085441
TCAAATC1517750.035.09409737
AGTCAAA1565500.034.0650835
CGTATTC212150.033.31221845
CAGTCAA1606400.033.25369334
AGTCGGA57000.032.84941
CGTTTGC210050.032.6490845
Produced by FastQC (version 0.11.5)