FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1792-04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1792-04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23429744
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC16049626.850104721588081TruSeq Adapter, Index 16 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCGCGTATGC1473330.6288288937343917TruSeq Adapter, Index 16 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2002900.040.42949345
TCGTATG1866650.039.77049344
CATCTCG1867950.039.6785340
CTCGTAT1883350.039.424443
ATCTCGT1886150.039.31713541
TCTCGTA1897100.039.1196842
CCATCTC1949700.038.00213639
TCCATCT1958000.037.8858638
GTCCATC2143300.037.74088337
CGTCCAT2163650.037.36063836
CCGTCCA2247700.036.01163535
CCCGTCC2321450.034.87019334
AGTCGGA74100.033.7121281
ACCCGTC2419950.033.5568533
CACCCGT2452350.033.13735632
TCACCCG2474750.032.85378631
GTCACCC2510550.032.4058430
AGTCACC2540600.032.0978229
CAGTCAC2641300.030.93456528
CCAGTCA2683800.030.46481727
Produced by FastQC (version 0.11.5)