FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1792-03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1792-03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25694918
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC21389498.32440484923906TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTCGTATGC9071113.5303128813254046TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATGC1461340.5687272479328402TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCGCGTATGC569480.22163137473332276TruSeq Adapter, Index 20 (97% over 37bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA261020.10158428993624342TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3598850.041.756745
TCGTATG3428850.041.05694644
CTCGTAT3472500.040.8043943
ATCTCGT3501150.040.61451741
TCTCGTA3501150.040.57459342
GATCTCG3516200.040.5731440
AGATCTC3532500.040.39929639
GAGATCT3562800.040.21107538
TAGAGAT2822850.038.13552536
AGAGATC2898400.037.42567437
GTAGAGA2905950.037.05117435
AGTCGGA113950.036.5574721
CGTAGAG3002400.035.84716434
TCGAGAT1255450.035.8256736
GTCGAGA1265100.035.53994835
ACGTAGA3146050.034.3019133
CGTCGAG1318600.034.07040834
CACGTAG3174750.033.9861532
TCACGTA3246450.033.3353431
CGAGATC1378350.032.7945937