Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n01_ds1792-03.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25694918 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC | 2138949 | 8.32440484923906 | TruSeq Adapter, Index 3 (97% over 37bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTCGTATGC | 907111 | 3.5303128813254046 | TruSeq Adapter, Index 20 (97% over 37bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATGC | 146134 | 0.5687272479328402 | TruSeq Adapter, Index 3 (97% over 37bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCGCGTATGC | 56948 | 0.22163137473332276 | TruSeq Adapter, Index 20 (97% over 37bp) |
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA | 26102 | 0.10158428993624342 | TruSeq Adapter, Index 3 (97% over 37bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 359885 | 0.0 | 41.7567 | 45 |
TCGTATG | 342885 | 0.0 | 41.056946 | 44 |
CTCGTAT | 347250 | 0.0 | 40.80439 | 43 |
ATCTCGT | 350115 | 0.0 | 40.614517 | 41 |
TCTCGTA | 350115 | 0.0 | 40.574593 | 42 |
GATCTCG | 351620 | 0.0 | 40.57314 | 40 |
AGATCTC | 353250 | 0.0 | 40.399296 | 39 |
GAGATCT | 356280 | 0.0 | 40.211075 | 38 |
TAGAGAT | 282285 | 0.0 | 38.135525 | 36 |
AGAGATC | 289840 | 0.0 | 37.425674 | 37 |
GTAGAGA | 290595 | 0.0 | 37.051174 | 35 |
AGTCGGA | 11395 | 0.0 | 36.557472 | 1 |
CGTAGAG | 300240 | 0.0 | 35.847164 | 34 |
TCGAGAT | 125545 | 0.0 | 35.82567 | 36 |
GTCGAGA | 126510 | 0.0 | 35.539948 | 35 |
ACGTAGA | 314605 | 0.0 | 34.30191 | 33 |
CGTCGAG | 131860 | 0.0 | 34.070408 | 34 |
CACGTAG | 317475 | 0.0 | 33.98615 | 32 |
TCACGTA | 324645 | 0.0 | 33.33534 | 31 |
CGAGATC | 137835 | 0.0 | 32.79459 | 37 |