FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1792-02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1792-02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23026489
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC22801459.902269512299508TruSeq Adapter, Index 18 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTATGC1956300.849586752022855TruSeq Adapter, Index 18 (98% over 50bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC320580.139222267016044TruSeq Adapter, Index 18 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG284040.12335358638479361TruSeq Adapter, Index 18 (100% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA275470.11963178580981235TruSeq Adapter, Index 18 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2767700.041.5680445
TCGTATG2595000.040.7431544
CATCTCG2615600.040.57573740
CTCGTAT2620250.040.45984343
ATCTCGT2633550.040.274441
CGCATCT2640400.040.21243338
TCTCGTA2638050.040.21072442
GCATCTC2635800.040.20928639
CCGCATC2962700.039.0519137
TCCGCAT3005450.038.44161636
GTCCGCA3103850.037.2693135
AGTCGGA88250.036.2376021
CGTCCGC3206500.036.0717634
ACGTCCG3323250.034.86612733
CACGTCC3392050.034.22527732
TCACGTC3414250.033.99944331
GTCACGT3451650.033.65704330
AGTCACG3479650.033.46129229
CAGTCAC3623800.032.1600528
CCAGTCA3671850.031.75704227