Basic Statistics
Measure | Value |
---|---|
Filename | HKKJCDRXX_l02_n01_ds1792-02.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23026489 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC | 2280145 | 9.902269512299508 | TruSeq Adapter, Index 18 (100% over 50bp) |
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTATGC | 195630 | 0.849586752022855 | TruSeq Adapter, Index 18 (98% over 50bp) |
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGCC | 32058 | 0.139222267016044 | TruSeq Adapter, Index 18 (100% over 49bp) |
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG | 28404 | 0.12335358638479361 | TruSeq Adapter, Index 18 (100% over 38bp) |
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCA | 27547 | 0.11963178580981235 | TruSeq Adapter, Index 18 (100% over 40bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 276770 | 0.0 | 41.56804 | 45 |
TCGTATG | 259500 | 0.0 | 40.74315 | 44 |
CATCTCG | 261560 | 0.0 | 40.575737 | 40 |
CTCGTAT | 262025 | 0.0 | 40.459843 | 43 |
ATCTCGT | 263355 | 0.0 | 40.2744 | 41 |
CGCATCT | 264040 | 0.0 | 40.212433 | 38 |
TCTCGTA | 263805 | 0.0 | 40.210724 | 42 |
GCATCTC | 263580 | 0.0 | 40.209286 | 39 |
CCGCATC | 296270 | 0.0 | 39.05191 | 37 |
TCCGCAT | 300545 | 0.0 | 38.441616 | 36 |
GTCCGCA | 310385 | 0.0 | 37.26931 | 35 |
AGTCGGA | 8825 | 0.0 | 36.237602 | 1 |
CGTCCGC | 320650 | 0.0 | 36.07176 | 34 |
ACGTCCG | 332325 | 0.0 | 34.866127 | 33 |
CACGTCC | 339205 | 0.0 | 34.225277 | 32 |
TCACGTC | 341425 | 0.0 | 33.999443 | 31 |
GTCACGT | 345165 | 0.0 | 33.657043 | 30 |
AGTCACG | 347965 | 0.0 | 33.461292 | 29 |
CAGTCAC | 362380 | 0.0 | 32.16005 | 28 |
CCAGTCA | 367185 | 0.0 | 31.757042 | 27 |