FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1792-01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1792-01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18262683
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC16184948.862301338746338TruSeq Adapter, Index 19 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGTATGC1076810.589623112879964TruSeq Adapter, Index 19 (97% over 42bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGCC239990.13141004528195557TruSeq Adapter, Index 19 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1940550.041.14424545
TCGTATG1859450.040.29837844
CTCGTAT1883750.039.95813843
ATCTCGT1895650.039.7869341
AATCTCG1901500.039.70948840
TCTCGTA1901600.039.6682542
AAATCTC1962100.038.501439
AAAATCT2004050.037.79538738
GAAAATC2140850.037.7376237
TGAAAAT2224400.036.31086736
GTGAAAA2238000.036.10830735
AGTCGGA64900.035.7168271
CGTGAAA2270200.035.6394734
ACGTGAA2325650.034.81101233
CACGTGA2340750.034.6018332
TCACGTG2363200.034.28549631
GTCACGT2383300.034.03787630
AGTCACG2398650.033.9091729
CAGTCAC2498100.032.5943628
CCAGTCA2535550.032.14488627
Produced by FastQC (version 0.11.5)