FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l02_n01_ds1792-00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l02_n01_ds1792-00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19454992
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC16515468.48906028848534TruSeq Adapter, Index 20 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATGC1404900.7221282846068505TruSeq Adapter, Index 20 (97% over 42bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC335470.1724338925454197TruSeq Adapter, Index 20 (97% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGC217160.11162173698143901TruSeq Adapter, Index 20 (100% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCA200710.10316632358419885TruSeq Adapter, Index 20 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2049850.040.2192145
TCGTATG1923150.039.48300644
CATCTCG1938300.039.1356440
CTCGTAT1944400.039.0833543
ATCTCGT1960500.038.72117641
TCTCGTA1963400.038.7005542
GCCATCT1978900.038.3236238
CCATCTC2014850.037.6342539
GGCCATC2218600.037.11602837
AGTCGGA80150.036.838571
TGGCCAT2264850.036.3330736
GTGGCCA2313800.035.59942235
CGTGGCC2383400.034.5792334
ACGTGGC2437300.033.9198533
CACGTGG2455500.033.71516832
TCACGTG2472400.033.5265731
GTCACGT2487500.033.3673730
AGTCACG2499450.033.2816629
CAGTCAC2613900.031.86831528
GTGACTG86850.031.431381
Produced by FastQC (version 0.11.5)