FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36739375
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5512001.5002977051188269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT13750.021.7615664
CGATCTA7600.018.35544235
AGCGATC8350.017.24572433
TAGGACG40750.016.9493084
GAGCGAT9200.014.42958632
GGACGTG45150.014.2511376
GTCCTAA45100.014.2351491
GACGTGA28100.013.7708947
GTCCTAC54150.013.561661
GCGATCT10000.013.50013234
TATACAC553900.013.35205145
CGAGCGA10050.013.20915531
GTAGGAC49700.012.9009773
GTATTCG5250.012.8722861
TTAGGAC41600.012.4385183
GTATCGC6100.012.1864671
CCGTGAT33750.011.5997527
GATCGGA10150.011.5338632
CGTCTAG9200.011.5081031
TACAGTG78550.011.3706565
Produced by FastQC (version 0.11.5)