FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb274.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb274.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34937716
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG476770.1364628414748119No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT136000.024.00515245
TAGGACG120350.017.7974384
CGATCTA12100.017.66461635
CTGACGC194550.016.82703844
GGACGTG133200.015.6244276
AGGACGT139900.015.6159395
CGACGAG14300.015.41899328
GTCCTAC169150.014.885551
TATACAC1471200.014.36662645
TCTGACG233300.013.99357343
ACGAGCG15300.013.97005730
GTAGGAC157200.013.711333
GTCCTAA130600.013.6576831
GACGTGA75100.013.3616757
TTTCTCG85100.012.74336341
CGTGATT91700.012.58676128
AGCGATC18000.012.49950533
GACGTGG87000.012.3615997
CCGTGAT93350.012.31608227
TCCTACA214850.012.184162
Produced by FastQC (version 0.11.5)