FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb273.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb273.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42290041
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG670170.1584699338551126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA27000.030.16551435
CGACGAG34050.024.31636628
ACGAGCG33900.024.29122230
GCGATCT35100.023.65294334
AGCGATC35200.023.52185633
TGACGCT176400.023.12452545
GAGCGAT39600.021.07883832
GCCGACG42550.019.9875326
GACGAGC43000.019.35981629
CCGACGA42950.019.27758627
TGCCGAC44600.018.76614425
TAGGACG106150.018.6733994
CGAGCGA45850.018.20549831
CGTCTAG27650.017.7567081
CTGACGC236350.017.29708544
GGACGTG123400.015.2425836
TCTGACG272000.015.03829543
CGTAATC19600.015.03797522
CTACGGT26850.014.07762313
AGGACGT146400.013.8775925
Produced by FastQC (version 0.11.5)