FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36535348
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT136000.023.45933545
CGATCTA12150.019.25836435
TAGGACG115600.018.1200074
CTGACGC188850.017.0133444
GGACGTG127050.015.7963486
AGGACGT137900.015.4018975
GACGTGA73550.014.6527377
TCTGACG220250.014.56739843
CGACGAG15800.014.52483928
TATACAC1351700.014.29186645
GTCCTAC158700.014.133751
CGTCTAG21250.013.4592171
ACGAGCG17050.013.32795430
GTCCTAA126400.013.2912781
GTAGGAC154100.013.2571353
CCGACGA18850.012.29402727
GCGATCT18900.012.26133634
TTAGGAC117550.012.2305153
TTATACA1823900.012.176949545
CTACGGT20300.011.969871513
Produced by FastQC (version 0.11.5)