FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39453259
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT154300.024.93467145
CGATCTA13650.020.60362635
TAGGACG123250.018.6201114
CTGACGC211650.018.2207444
ACGAGCG17300.016.64684330
CGACGAG17500.016.4565928
GGACGTG132000.016.3460856
CGTCTAG22250.016.0938191
TCTGACG249200.015.42102843
AGGACGT148200.015.3335335
GTCCTAC182750.015.0470111
GACGTGA78050.014.2115697
TATACAC1423350.014.14608645
TACGCCG8650.013.7857015
AGCGATC20750.013.77058933
GTAGGAC166900.013.669443
GTCCTAA134350.013.2093221
CGAGCGA21900.013.15024631
GCGATCT21400.013.03689834
GACGTGG85900.012.4937627
Produced by FastQC (version 0.11.5)