FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39941420
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT155150.024.23266445
CGATCTA14900.022.04614835
TAGGACG122450.018.7048664
CTGACGC214250.017.61116844
CGACGAG18600.017.05589528
ACGAGCG19700.016.56037330
GGACGTG136850.015.9968216
AGGACGT150100.015.3191915
TCTGACG252600.014.91960843
GTCCTAC169050.014.4272511
GCGATCT22800.014.30867134
GACGTGA79150.014.241397
CGTCTAG20750.014.2175081
TATACAC1421650.014.049149545
GTAGGAC166950.013.9751973
AGCGATC22900.013.95142233
GAGCGAT24600.013.07884132
CGAGCGA26700.012.47150331
CGTAATC16300.012.14705622
TGTAGGA220800.012.1104042
Produced by FastQC (version 0.11.5)