FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37649660
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG487790.12956026694530576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT174100.026.29916245
CTGACGC248750.018.43390544
TAGGACG132800.017.9247884
CGATCTA13150.017.28070635
TCTGACG288650.015.84681843
GGACGTG145700.015.7046366
AGGACGT155200.015.5986495
GTCCTAC173700.014.7937261
GACGTGA85600.014.7453277
ACGAGCG15700.014.18739230
CGACGAG15450.014.125710528
GTAGGAC163100.013.5188133
TATACAC1855900.013.32595745
CGTCTAG19150.012.9364661
GTCCTAA144300.012.7823081
TTAGGAC127650.012.619983
AGCGATC19350.012.44140133
TCCTACA229000.012.1978512
CTACAGT233050.011.7781534
TGTAGGA218500.011.7744822
Produced by FastQC (version 0.11.5)