FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb268.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb268.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36058521
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT176150.027.83225845
CTGACGC245800.019.9731344
TAGGACG120600.018.227024
TCTGACG288850.016.99634743
GGACGTG131550.016.2993566
AGGACGT141500.015.6461355
GACGTGA77850.014.7394067
CGATCTA10600.014.43351435
GTCCTAC163100.014.3036381
GTAGGAC154850.013.7305843
TATACAC1959150.013.4378345
GTCCTAA137550.012.7858961
TTAGGAC124800.012.5476693
GACGTGG82400.012.4510527
ACGAGCG12700.012.22408130
ATCTGAC415900.011.97738442
ATACACA1538900.011.94790645
CTACAGT214950.011.8174814
TAGACGC29400.011.6322764
TCCTACA222150.011.5394612
Produced by FastQC (version 0.11.5)