FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb267.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb267.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52814090
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG914690.17319052548287775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA38900.030.30720935
CGACGAG45650.026.6146128
ACGAGCG45850.026.30220630
GCGATCT46600.025.87873834
AGCGATC47800.025.13492633
TGACGCT285750.023.71618845
GAGCGAT53100.022.79576132
CGAGCGA57200.021.31913431
GACGAGC58800.020.6242529
GCCGACG59700.020.53943426
CCGACGA60500.020.30506527
TGCCGAC62100.019.4919925
TAGGACG138800.018.2684444
CTGACGC378400.017.92719744
TCTGACG433950.015.64788843
GGACGTG160100.015.2758496
CGTCTAG32900.014.9917331
CTGCCGA87200.014.13964824
AGGACGT188400.014.0440885
GTCCTAC213050.013.5416381
Produced by FastQC (version 0.11.5)