FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb266.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb266.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52796060
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT252800.024.8136645
TAGGACG185800.018.139844
CTGACGC357350.017.60427344
CGATCTA19250.017.41487735
ACGAGCG19700.017.35981630
GGACGTG201300.016.0612856
AGGACGT220800.015.3866895
CGACGAG23400.014.80718928
TCTGACG424000.014.78393343
GACGTGA117600.014.6359117
CGTCTAG27300.014.1897081
GTCCTAC251050.014.0398261
TATACAC2348450.013.65526445
GTAGGAC239300.013.5860273
AGCGATC26100.013.27534833
GCGATCT27150.013.0105234
CGAGCGA27950.012.47721431
GACGTGG127350.012.2256747
GTCCTAA198350.012.0813941
TTAGGAC183750.012.0485583
Produced by FastQC (version 0.11.5)