FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n02_mb265.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n02_mb265.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50505906
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG684000.1354297059832963No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA23500.026.99898135
TGACGCT247900.024.78681845
ACGAGCG28100.022.89955930
CGACGAG29250.022.15307628
GCGATCT31250.020.51922634
AGCGATC33800.018.97117833
GAGCGAT34050.018.83194432
TAGGACG154450.018.6899034
CTGACGC339450.018.09516144
CGAGCGA37900.017.39385431
GACGAGC39750.016.6409329
CCGACGA39750.016.52772527
GGACGTG169350.016.3015026
CGTCTAG29600.016.2823331
GCCGACG40800.015.99205926
TCTGACG395550.015.517385543
AGGACGT190650.015.3417465
GACGTGA100250.014.6553517
TGCCGAC45200.014.53484925
GTCCTAC225100.014.2872041
Produced by FastQC (version 0.11.5)