FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36739375
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC60600.022.90893645
TAGGACG36250.019.1802434
GGACGTG40050.017.0233696
GAAATCT84500.015.8972214
ACCGATC7700.015.77917134
AAATCTC88000.015.674125
CGTCTAG6750.015.3374171
CTCCGAG92050.015.15508844
TCTCCGA93450.014.85617843
GTAGGAC51600.014.5647013
GTCCTAC52600.014.2908781
CTAGACG8100.013.8896013
CGACGAA3600.013.74952523
TATACAC555100.013.39248645
TACGGTG7400.013.37777114
CTACGGT7400.013.37777113
GACGTGA25600.013.1831047
ATCTCCG106550.013.177175542
GACGTGG26450.013.0146387
AGATCGG9850.012.5667971
Produced by FastQC (version 0.11.5)