FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb274.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb274.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34937716
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC139100.023.38923645
TAGGACG120350.019.2563294
TCGCGTA3700.017.63482544
GGACGTG129250.017.2514696
AGGACGT137950.016.9137555
GACGTGA75000.016.0498337
GTCCTAC167150.015.1603151
GTAGGAC154800.015.1017823
CTCCGAG219350.014.92451644
TATACAC1479800.014.34087645
GTCCTAA130550.013.6701271
TCTCGTA14400.013.59351244
TCTCCGA243550.013.34019143
CGTCTAG18600.013.3093231
GACGTGG83750.012.9222557
CTCGTAT14400.012.81227645
TGTAGGA202350.012.7455232
TCCTACA217550.012.67223552
TTAGGAC119900.012.5354773
GGTACGA12550.012.37030118
Produced by FastQC (version 0.11.5)