FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb273.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb273.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42290041
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA26550.031.18643837
CGCTACG29100.029.07174934
ACGCTAT29050.028.6575238
CGCTATC29100.028.60827639
CTCGTAT25500.027.44078445
TCTCGTA25600.026.98206944
TATCTCG28450.023.40951742
GCTACGC36500.023.05454635
TCCGAGC189450.021.70990445
ACGCTAC41200.021.13440733
TCGCGTA7900.021.07567444
ATCTCGT33250.020.50378243
CGTCTAG28150.020.3068981
CTACGCT43350.019.35965536
TAGGACG100250.017.5512264
GACGCTA53150.016.84830332
ACGAGAC52450.016.21515528
CTACGGT29900.015.50139613
TACGGTG29600.015.43046814
GAGACGC57100.015.24930930
Produced by FastQC (version 0.11.5)