FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb272.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb272.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36535348
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC147000.022.14760445
TAGGACG114600.019.9672584
CGTCTAG23350.017.8303171
GGACGTG124650.017.3466016
AGGACGT139000.016.3651375
GACGTGA75500.015.436847
GTAGGAC150850.014.5575533
CTCCGAG225550.014.51426844
GTCCTAC165900.014.3520371
TATACAC1369800.014.24254645
TCTCCGA237400.013.70489243
TCTCGTA14500.013.65493644
TTGACGG26150.013.59448531
CGACGAA10750.013.18581823
TAGACGC31200.012.9807584
CTCGTAT15650.012.93909145
GACGTGG78250.012.852837
TTAGGAC121900.012.7728053
GTCCTAA122650.012.7340391
CTACGGT20300.012.30274213
Produced by FastQC (version 0.11.5)