FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb271.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb271.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39453259
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC162200.023.85910245
TCGCGTA4250.022.23498544
TAGGACG124800.019.3450284
CGTCTAG25700.017.4256041
GGACGTG138400.016.8588526
AGGACGT152750.015.98215
CTCCGAG244100.015.87237444
TCTCCGA261450.014.77628743
GTCCTAC179150.014.6093471
GACGTGA82100.014.49747
GTAGGAC171850.014.3367053
TATACAC1430150.014.09465645
GACGTGG88550.013.365187
TAGACGC34350.013.3624974
ATCGCGT6950.013.27341743
TCCGCAA22950.013.23511133
ATCTCCG291600.013.22534442
GTCCTAA137450.012.950891
CTACGGT24750.012.81802113
TACGGTG25400.012.66716514
Produced by FastQC (version 0.11.5)