FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39941420
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC166300.022.70255945
TAGGACG119750.018.2818894
CGTCTAG24150.016.027881
GGACGTG134500.015.5410146
TCGCGTA4850.015.30903544
CTACGGT22050.015.20382113
TAGACGC35600.015.1685834
CTAGACG25300.015.118623
AGGACGT146400.015.0308565
CTCCGAG254600.014.86425644
GACGTGA78100.014.6925237
GTCCTAC173300.014.5310261
TACGGTG23200.014.45018314
TCTCCGA262850.014.3295643
TATACAC1431250.013.95329845
CTCGTAT19700.013.24850445
TTAGGAC123600.013.1068333
GTAGGAC159700.013.0604633
TTGACGG28900.012.92371831
ATCTCCG299100.012.57029242
Produced by FastQC (version 0.11.5)