FastQCFastQC Report
Sat 22 Feb 2020
HKKJCDRXX_l01_n01_mb269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKKJCDRXX_l01_n01_mb269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37649660
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC180350.025.58741645
TAGGACG129850.018.3846824
CTCCGAG282950.016.35691844
GGACGTG136200.016.3547176
AGGACGT150050.015.6848615
TCTCCGA303900.015.19252343
GTCCTAC184100.014.7665641
CGTCTAG19300.014.575351
GACGTGA82150.014.4612237
GTAGGAC165500.014.3973243
ATCTCCG343200.013.42659642
TATACAC1868450.013.275002545
GTCCTAA140550.013.0014421
GACGTGG87250.012.5844157
CTCGTAT15100.012.51639545
TTAGGAC133300.012.3556263
TGTAGGA214800.012.3312892
TCCTACA242850.012.2413982
TTTCTCG92350.012.23066441
TTCTCGC92350.012.08448142
Produced by FastQC (version 0.11.5)